Efficacy case studies
In Vitro Assessment of the Anti-Oxidant Potential of Five Active Cosmetic Ingredients in the Development of an Anti-Ageing Skincare Formulation
Anti-oxidant activity forms a vital part of the body’s natural defence system against reactive oxygen species (ROS), which are formed as a by-product of metabolism and can be harmful to cells. ROS levels in the skin are increased dramatically by a variety of internal and external factors including stress, the ageing process and exposure to air pollutants. Anti-oxidant cosmetic ingredients may therefore support a variety of claims including anti-pollution and anti-ageing and have gained significant popularity. The OxiSelect™ test measures anti-oxidant activity against hydroxyl, peroxyl and other ROSin human skin cell (epidermal keratinocyte) cultures, indicating the potential of active cosmetic ingredients to protect against the effects of ageing and pollution. The aim of this study was to assess the anti-oxidant potential of five active ingredients (designated codes A, B, C, D, E), alone and in combination (A+B; A+C; A+B+C; A+B+D; A+B+E in equal concentrations)
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In Vitro Assessment of Mildness to the Eye of a Range of 29 Babycare Formulations
Mildness to the eye (ie lack of irritation potential) is a desirable characteristic for a wide range of cosmetic and personal care products, and particularly important in the context of babycare formulations. Reconstructed human corneal models utilise tissue engineering technology to provide a powerful tool for the generation of data supporting No Tears-like claims, giving a higher level of confidence in product safety prior to human trials. In this study, the human corneal model, EpiOcular™, was used to assess the eye irritation potential of a range of babycare formulations (15 bath products, 8 body washes and 6 shampoos). The products were applied to the cornea surface and cellular damage was assessed using a metabolic dye (MTT). Results were correlated with a classification of eye irritation potential (Severe, Moderate, Mild or Minimal / Non-Irritant) using a validated statistical model.
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In Vitro Assessment of Mildness to the Skin of a Range of 12 Surfactants Used in Personal Care Products
Mildness to the skin (ie lack of irritation potential) is a desirable characteristic for a wide range of cosmetic and personal care products, including babycare formulations and products designed for sensitive skin. Reconstructed human skin models utilise tissue engineering technology to provide a powerful tool for the generation of data supporting claims such as mild and suitable for sensitive skin, giving a higher level of confidence in product safety prior to human trials. In this study, the human skin model, EpiDerm™, was used to assess the skin irritation potential of 12 surfactants commonly used in personal care products. The products were applied to the skin surface and cellular damage was assessed using a metabolic dye (MTT). Results were correlated with a classification of skin irritation potential (Severe, Moderate, Mild to Moderate, Mild, or Minimal / Non-Irritant) using a validated statistical model.
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